Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43.
10.4142/jvs.2010.11.2.165
- Author:
Hyun Kyoung LEE
1
;
Byoung Hee LEE
;
Seung Hyeok SEOK
;
Min Won BAEK
;
Hui Young LEE
;
Dong Jae KIM
;
Yi Rang NA
;
Kyoung Jin NOH
;
Sung Hoon PARK
;
Dutta Noton KUMAR
;
Hiroaki KARIWA
;
Mina NAKAUCHI
;
Suk Jin HEO
;
Jae Hak PARK
Author Information
1. Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. pjhak@snu.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
cross-reactivity;
HCoV 229E;
HCoV OC43;
recombinant nucleocapsid protein;
SARS
- MeSH:
Antibodies, Viral/*immunology;
Blotting, Western;
Coronavirus 229E, Human/*immunology;
Coronavirus OC43, Human/*immunology;
Cross Reactions;
Humans;
Nucleocapsid Proteins/genetics/*immunology;
Recombinant Proteins/immunology;
SARS Virus/genetics/*immunology;
Severe Acute Respiratory Syndrome/diagnosis/*immunology
- From:Journal of Veterinary Science
2010;11(2):165-167
- CountryRepublic of Korea
- Language:English
-
Abstract:
Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV OC43 were determined by Western blotting. The Abs produced reacted with recombinant SARS virus nucleocapsid protein, but not with HCoV 229E or HCoV OC43.
