Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein.
10.4142/jvs.2010.11.2.169
- Author:
Mingeun SAGONG
1
;
Choi Kyu PARK
;
Seong Hee KIM
;
Sung Up MOON
;
Seong Cheol CHO
;
Changhee LEE
Author Information
1. Department of Microbiology, College of Natural Sciences, Kyungpook National University, Daegu 702-701, Korea. changhee@knu.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
diagnostic reagent;
nucleocapsid protein;
PRRSV;
stable BHK cell line
- MeSH:
Animals;
Antibodies, Viral/analysis/immunology;
Blotting, Western/veterinary;
Cell Line;
Cricetinae;
Female;
Genotype;
Nucleocapsid Proteins/genetics/*immunology;
Porcine Reproductive and Respiratory Syndrome/diagnosis/*immunology;
Porcine respiratory and reproductive syndrome virus/genetics/*immunology;
Swine;
Transfection/veterinary
- From:Journal of Veterinary Science
2010;11(2):169-171
- CountryRepublic of Korea
- Language:English
-
Abstract:
Despite global efforts to control porcine reproductive and respiratory syndrome virus (PRRSV) infection, the virus continues to cause economic problems in the swine industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively express the nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell lines were found to react well with N-specific antibodies as well as the hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, the data implicate a potential usefulness for the newly generated stable cell lines as a diagnostic reagent for PRRSV serology.
