Bone regeneration effects of human allogenous bone substitutes: a preliminary study.
10.5051/jpis.2010.40.3.132
- Author:
Deok Won LEE
1
;
Ki Tae KOO
;
Yang Jo SEOL
;
Yong Moo LEE
;
Young KU
;
In Chul RHYU
;
Chong Pyoung CHUNG
;
Tae Il KIM
Author Information
1. Department of Periodontology and Dental Research Institute, Seoul National University College of Dentistry, Seoul, Korea. periopf@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Bone Substitutes;
Osteogenesis;
Transplantation;
X-Ray Microtomography
- MeSH:
Animals;
Bone Matrix;
Bone Regeneration;
Bone Substitutes;
Humans;
Osteogenesis;
Rabbits;
X-Ray Microtomography
- From:Journal of Periodontal & Implant Science
2010;40(3):132-138
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The purpose of this study was to compare the bone regeneration effects of cortical, cancellous, and cortico-cancellous human bone substitutes on calvarial defects of rabbits. METHODS: Four 8-mm diameter calvarial defects were created in each of nine New Zealand white rabbits. Freeze-dried cortical bone, freeze-dried cortico-cancellous bone, and demineralized bone matrix with freeze-dried cancellous bone were inserted into the defects, while the non-grafted defect was regarded as the control. After 4, 8, and 12 weeks of healing, the experimental animals were euthanized for specimen preparation. Micro-computed tomography (micro-CT) was performed to calculate the percent bone volume. After histological evaluation, histomorphometric analysis was performed to quantify new bone formation. RESULTS: In micro-CT evaluation, freeze-dried cortico-cancellous human bone showed the highest percent bone volume value among the experimental groups at week 4. At week 8 and week 12, freeze-dried cortical human bone showed the highest percent bone volume value among the experimental groups. In histologic evaluation, at week 4, freeze-dried cortico-cancellous human bone showed more prominent osteoid tissue than any other group. New bone formation was increased in all of the experimental groups at week 8 and 12. Histomorphometric data showed that freeze-dried cortico-cancellous human bone showed a significantly higher new bone formation percentile value than any other experimental group at week 4. At week 8, freeze-dried cortical human bone showed the highest value, of which a significant difference existed between freeze-dried cortical human bone and demineralized bone matrix with freeze-dried cancellous human bone. At week 12, there were no significant differences among the experimental groups. CONCLUSIONS: Freeze-dried cortico-cancellous human bone showed swift new bone formation at the 4-week healing phase, whereas there was less difference in new bone formation among the experimental groups in the following healing phases.
