Comparison of Total and IgG ABO Isoagglutinin Titers in ABO-Incompatible Organ Transplant Patients: Analysis of Data from a University Hospital Over the Last 5 Years
10.17945/kjbt.2022.33.3.1 4 5
- Author:
Jung-Ah KIM
1
;
Jeong Won SHIN
;
Do Lee LEE
;
Suyeon PARK
;
Jieun KIM
;
Hae In BANG
Author Information
1. Department of Laboratory Medicine, Soonchunhyang University Seoul Hospital, Seoul, Korea
- Publication Type:Original Article
- From:Korean Journal of Blood Transfusion
2022;33(3):145-153
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Measurement of ABO isoagglutinin titers is important for patients who have received an ABO-incompatible organ transplant. Specifically, IgG isoagglutinin is essential for predicting graft outcomes in kidney transplantation, but many laboratories measure only the total isoagglutinin taking into consideration time and labor efficiency. In this study, we propose a useful method for predicting IgG isoagglutinin by analyzing the mathematical relationship between total and IgG isoagglutinin titers. Furthermore, the effects of patients’ characteristics of isoagglutinin were also analyzed.
Methods:From January 2017 to April 2022, the results of 3,676 total/IgG isoagglutinin titers of 65 patients who underwent liver and kidney transplantation were analyzed. Isoagglutinin titration was performed using the column agglutination technique with serially diluted serum samples and dithiothreitol was added for measuring IgG isoagglutinin. A generalized estimation equation (GEE) and the Deming regression were used to analyze the relationship and agreement of total/IgG isoagglutinin titers.
Results:In A, B, and O types, total isoagglutinin titers were 1.6 (2^67.1×10–2 ), 1.9 (2^95.1×10–2 ), and 2.0 (2^98.5×10–2 ) times higher than IgG isoagglutinin, respectively, and the agreement between the two tests was high in all blood types. The blood types affected total/IgG isoagglutinin titers (P＜0.05, GEE), but age, sex, and the type of transplanted organs did not have a statistically significant effect.
Conclusion:We concluded that if measuring IgG isoagglutinin titers in ABO-incompatible organ transplant patients is not feasible, the relationship between total and IgG isoagglutinin, viz. total isoagglutinin titers=1.6, 1.9 or 2.0×IgG isoagglutinin for the A, B, and O type blood groups respectively can be used as an auxiliary means of prediction.
