Expression of FMD virus-like particles in yeast Hansenula polymorpha and immunogenicity of combine with CpG and aluminum adjuvant
- Author:
Jianhui ZHANG
1
;
Jun GE
;
Juyin LI
;
Jianqiang LI
;
Yong ZHANG
;
Yinghui SHI
;
Jiaojiao SUN
;
Qiongjin WANG
;
Xiaobo ZHANG
;
Xingxu ZHAO
Author Information
- Publication Type:Original Article
- From:Journal of Veterinary Science 2023;24(1):e15-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Inactivated vaccines are limited in preventing foot-and-mouth disease (FMD) due to safety problems. Recombinant virus-like particles (VLPs) are an excellent candidate for a novel vaccine for preventing FMD, given that VLPs have similar immunogenicity as natural viruses and are replication- and infection-incompetent.
Objectives:The 3C protease and P1 polyprotein of type O FMD virus (FDMV) was expressed in yeast Hansenula polymorpha to generate self-resembling VLPs, and the potential of recombinant VLPs as an FMD vaccine was evaluated.
Methods:BALB/c mice were immunized with recombinant purified VLPs using CpG oligodeoxynucleotide and aluminum hydroxide gel as an adjuvant. Cytokines and lymphocytes from serum and spleen were analyzed by enzyme-linked immunosorbent assay, enzyme-linked immunospot assay, and flow cytometry.
Results:The VLPs of FMD were purified successfully from yeast protein with a diameter of approximately 25 nm. The immunization of mice showed that animals produced high levels of FMDV antibodies and a higher level of antibodies for a longer time. In addition, higher levels of interferon-γ and CD4 + T cells were observed in mice immunized with VLPs.
Conclusions:The expression of VLPs of FMD in H. polymorpha provides a novel strategy for the generation of the FMDV vaccine.
