Effect of Homocysteine on the Expression of Matrix Metalloproteinase-9 and Apoptosis in Trabecular Meshwork Cells
10.3341/jkos.2023.64.3.239
- Author:
Jeong Yeub KIM
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Daegu Catholic University School of Medicine, Daegu, Korea
- Publication Type:Original Article
- From:Journal of the Korean Ophthalmological Society
2023;64(3):239-244
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Purpose:In retinal endothelial cells, homocysteine (Hcy) activates matrix metalloproteinase (MMP)-9, which results in apoptosis. This study investigated these effects of Hcy in human trabecular meshwork cells (HTMC).
Methods:HTMC cultures using 5 mM low or 20 mM high glucose (HG)-containing media were exposed to 100 μM Hcy for 3 days. Cell viability was assessed with the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay. The MMP-9 and tissue inhibitor of MMP (TIMP)-1 levels were measured by western blotting and the degree of apoptosis was analyzed with flow cytometry using Annexin-propidium iodide double staining.
Results:Exposure to Hcy in HG decreased cell viability compared to HG alone (p = 0.036). Compared to HG alone, co-exposure to Hcy with HG decreased the TIMP-1 levels, albeit not significantly (p = 0.094), and did not affect the MMP-9 levels (p = 0.413). In addition, co-exposure to Hcy with HG produced no difference in the degree of apoptosis compared to HG alone (p = 0.437).
Conclusions:Unlike in retinal endothelial cells, Hcy did not affect the activities of TIMP-1, MMP-9, or the degrees of apoptosis significantly in HTMC. Thus, the effects Hcy may be limited in HTMC.
