Rapid and easy detection of Helicobacter pylori by in situ hybridization.
10.3346/jkms.1999.14.1.15
- Author:
Chang Soo PARK
1
;
Jin KIM
Author Information
1. Department of Pathology, Chonnam National University School of Medicine, Kwangju, Korea. cspark@chonnam.chonnam.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Helicobacter pylori;
In situ hybridization;
RNA, ribosomal;
Biopsy
- MeSH:
Helicobacter Infections/pathology;
Helicobacter Infections/microbiology*;
Helicobacter pylori/isolation & purification*;
Helicobacter pylori/genetics;
Human;
In Situ Hybridization/methods*;
Oligonucleotide Probes;
RNA, Bacterial/analysis;
RNA, Ribosomal, 16S/analysis;
Sensitivity and Specificity;
Silver Staining/methods;
Time Factors
- From:Journal of Korean Medical Science
1999;14(1):15-20
- CountryRepublic of Korea
- Language:English
-
Abstract:
Various in situ hybridization (ISH) methods have been used to identify Helicobacter pylori, a causative organism responsible for chronic gastritis and peptic ulcer disease, but they were hard to perform and time consuming. To detect H. pylori in a rapid and easily reproducible way, we developed synthetic biotinylated oligonucleotide probes which complement rRNA of H. pylori. Formalin-fixed and paraffin-embedded tissues from 50 gastric biopsy specimens were examined. Using a serologic test and histochemical stain (Warthin-Starry silver stain and/or Giemsa stain) as a standard, 40 of them were confirmed to be H. pylori-positive. Our ISH was quickly carried out within one hr and results were compared with those obtained from immunohistochemical stain. The ISH produced a positive reaction in 38 of 40 cases (95%). All H. pylori-negative cases failed to demonstrate a positive signal. The ISH has a sensitivity comparable to those of conventional histochemical and immunohistochemical stain, and has high specificity. In conclusion, ISH with a biotinylated oligonucleotide probe provides a useful diagnostic method for detecting H. pylori effectively in routinely processed tissue sections.
