Single-cell transcriptomics reveals tissue architecture in ovarian carcinosarcoma
- Author:
Junfen XU
1
;
Weiguo LU
Author Information
- Publication Type:Young Doctor Session
- From:Journal of Gynecologic Oncology 2022;33(S1):S4-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:Ovarian carcinosarcoma (OCS) is one of rarest and most challenging histologic subtype of ovarian cancer. It features remarkable cellular heterogeneity. Using single-cell RNA sequencing (scRNA-seq), we characterize the cellular composition of OCS and identify their molecular characteristics.
Methods:We applied scRNA-seq to resected primary OCS for the in-depth analysis of tumor cells and the tumor microenvironment. Immunohistochemistry staining was used for validation.
Results:Malignant epithelial and fibroblast cells displayed a high-degree of intratumoral heterogeneity. We revealed that certain epithelial cell subclusters had high levels of drug resistance scores and many active metabolic pathways. Furthermore, γδ T cells exhibited enriched interferon (IFN)-γ and IFN-α response characteristics. Analyzing ligand-receptor interaction pairs between cell types, we identified broadly interacting cells and observed an interaction between the ANXA1+ epithelial population and FPR1+/FPR3+ myeloid cells.
Conclusion:Our findings provide a single-cell transcriptomic signature of human OCS and present a well-established resource for elucidating OCS diversity.
