Sperm DNA fragmentation in consecutive ejaculates from patients with cancer for sperm cryopreservation
- Author:
Seul Ki KIM
1
;
Haerin PAIK
;
Jung Ryeol LEE
;
Byung Chul JEE
Author Information
- Publication Type:Original Article
- From:Clinical and Experimental Reproductive Medicine 2022;49(3):196-201
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:This prospective consecutive study investigated the variation in sperm DNA fragmentation (SDF) in multiple semen samples from patients with cancer.
Methods:Eighty-one patients with various cancers underwent multiple semen collections on 3 consecutive days for sperm cryopreservation prior to cancer treatment. A commercial Halosperm kit was used to measure SDF. Within- and between-subject coefficients of variation were estimated via random-effects analysis of variance to assess the consistency of semen parameters and SDF. Intraclass correlation coefficients (ICCs) were calculated to assess the magnitude of the between-subject component of variance relative to the total variance.
Results:The volume of semen in the day-2 and day-3 samples was significantly lower compared with the day-1 sample. Most parameters showed high ICC values, suggesting that within-subject fluctuations were small relative to the between-subject variability. The highest ICC values were identified for the SDF (ICC, 0.68; 95% confidence interval [CI], 0.45–0.84) and semen volume (ICC, 0.67; 95% CI, 0.45–0.84).
Conclusion:Our findings showed that repeated ejaculates from patients with cancer had stable SDF levels.
