Prevalence of Specific Clone of Salmonella enterica Serovar Typhimurium Clones in the Swine Population of Kyungpook Province During 1998 to 2000.
- Author:
Sung Yong SEOL
1
;
Ki Young KIM
;
Young Sook JEONG
;
Hee Young KANG
;
Hak Sun YU
;
Bong Hwan KIM
;
Dong Taek CHO
;
Yoo Chul LEE
;
Je Chul LEE
Author Information
1. Department of Microbiology, School of Medicine, Kyungpook National University, Daegu 700-422, Korea. syseol@knu.ac.kr
- Publication Type:Original Article
- Keywords:
Epidemiology;
Salmonella enterica serovar Typhimurium;
PFGE, plasmid;
antimicrobial resistance
- MeSH:
Clone Cells*;
Electrophoresis, Gel, Pulsed-Field;
Epidemiology;
Gyeongsangbuk-do*;
Plasmids;
Prevalence*;
Salmonella enterica*;
Salmonella*;
Streptomycin;
Swine*;
Tetracycline
- From:Journal of Bacteriology and Virology
2005;35(2):87-92
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
A total of 40 Salmolella enterica serovar Typhimurium (S. typhimurium) strains were isolated from clinical specimens of swine at 10 farms in Kyungpook province from 1998 to 2000. We investigated the clonal relationship of S. typhimurium isolates by antimicrobial susceptibility, plasmid profile, and Southern hybridization analysis with tetA, and pulsed-field gel electrophoresis (PFGE). All S. typhimurium isolates showed identical biochemical characteristics and were resistant to tetracycline, streptomycin, and sulfamehtoxazole. They were classified into 5 groups by antimicrobial resistance patterns. S. typhimurium isolates carried 3 to 5 plasmids and were classified into 5 groups by plasmid profiles. Southern hybridization showed that tetA gene was located in 21 kb of plasmid. S. typhimurium isolates from 9 different farms showed identical or similar PFGE patterns, which indicates clonal origin of the strains. All S. typhimurium isolates, except one isolate from 1998, seemed to belong to be one clone by the combination of three epidemiological typing methods. These data demonstrated that a specific clone of Salmolella enterica serovar Typhimurium was widely spread in swine farms in Kyungpook province.
