Effect of silencing E6-associated protein on level of p53 protein in human papilloma virus negative cervical cancer cells
10.13200/j.cnki.cjb.003812
- VernacularTitle:沉默E6相关蛋白对人乳头瘤病毒阴性宫颈癌细胞中p53蛋白表达水平的影响
- Author:
XIE Yi-hang
;
GUO Yu-wei
;
SUN Bo-xuan
;
XIN Yang
;
YU Jia-min
;
ZHAO Chun-yan
- Publication Type:Journal Article
- Keywords:
E6-associated protein(E6AP);
Human papilloma virus(HPV);
Cervical cancer;
C33A cells;
p53 protein
- From:
Chinese Journal of Biologicals
2023;36(2):163-165+171
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of silencing E6-associated protein(E6AP)on the level of p53 protein in human papilloma virus(HPV)negative cervical cancer cells(C33A cells).Methods The siRNA sequence silencing E6AP(siE6AP)and silencing control disordered siRNA sequence(siControl)were transfected into C33A cells with the mediation of LipofectamineTM2000 transfection reagent respectively.The silencing effect of siRNA on E6AP and the expression of p53and cleaved-caspase-3 proteins were detected by Western blot.Results The levels of E6AP protein in C33A cells of siE6AP group were significantly lower(t =-4.597,P<0.05),while the levels of p53 and cleaved-caspase-3 proteins were significantly higher than those of siControl group(t = 4.533 and 7.099 respectively,each P<0.05).Conclusion Silencing of E6AP significantly increased the expression of p53 protein in C33A cells,indicating that silencing of E6AP may restore the activity and function of p53 protein in C33A cells.
- Full text:沉默E6相关蛋白对人乳头瘤病毒阴性宫颈癌细胞中p53蛋白表达水平的影响.pdf
