Pharmacodynamics of human interferon α1b against severe acute respiratory syndrome coronavirus 2 Omicron strain in vitro
10.13200/j.cnki.cjb.003813
- VernacularTitle:人干扰素α1b体外抗SARS-CoV-2 Omicron株的药效
- Author:
LIU Lin-lin
1
;
LI Yu-wei
;
ZOU Yong
;
ZHANG Xue-mei
;
LU Jia
;
LIU Xiao-ke
;
WANG Ze-yun
;
LIU Yu-lin
;
LIU Jing-hui
Author Information
1. Changchun Institute of Biological Products Co.,Ltd.
- Publication Type:Journal Article
- Keywords:
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2);
Omicron strain;
Human interferon α1b;
Pharmacodynamics
- From:
Chinese Journal of Biologicals
2023;36(2):158-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the pharmacodynamics of human interferon(IFN)α1b against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron strain in vitro.Methods Total four drugs human IFNα1b bulk,human IFNα1b eye drops,human IFNα1b spray and Remdesivir were detected for cytotoxicity by CCK-8 assay.The inhibitory effect of human IFNα1b on SARS-CoV-2 Omicron strains(BA.5/BA.2/BA.1)was determined by qPCR.Results Human IFNα1b bulk of the maximum concentration(1 × 107IU/mL)and Remdesivir of the maximum concentration(150 μmol/L)did not achieve half cytotoxicity to Vero cells;The median cytotoxicity concentrations(CC_(50))of human IFNα1b eye drops and human IFNα1b sprays were 29 958 and 37 550 IU/mL,respectively,showing toxicity to Vero cells.The median effective concentrations(EC_(50))of human IFNα1b against virus strains BA.1,BA.2 and BA.5 after incubation for 2 h in advance were 9.30,13.38 and 12.33 IU/mL and those of Remdesivir were 0.314 7,0.291 0 and0.300 3 μmol/L.When incubation with virus simultaneously,the EC_(50)of human IFNα1b to BA.1,BA.2 and BA.5 were19.68,10.91 and 18.84 IU/mL and those of the control drug Remdesivir were 0.320 5,0.274 4 and 0.304 1 μmol/L,respectively.Conclusion At the cell level in vitro,human IFNα1b of very low activity showed a good inhibitory effect on SARS-CoV-2 Omicron strain,which was expected to be a clinical specific drug for the treatment of SARS-CoV-2 Omicron strain infection.
- Full text:人干扰素α1b体外抗SARS⁃CoV⁃2Omicron株的药效.pdf
