Clinical Application of the Next Generation Sequencing for Molecular Classification in Endometrial Carcinomas
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.2023.0205
- VernacularTitle:二代测序在子宫内膜癌分子分型的临床应用
- Author:
Ye LIU
1
;
Xiao-yun LIU
1
;
Shu-mei YAN
2
;
Ya-kang LONG
1
;
Hai-yun WANG
3
;
Fang WANG
1
Author Information
1. Department of Molecular Diagnostics， Sun Yat-sen University Cancer Center， Guangzhou 510060， China
2. Department of Pathology， Sun Yat-sen University Cancer Center， Guangzhou 510060， China
3. Department of Pathology， Guangzhou Women and Children's Medical Center， Guangzhou 510623， China
- Publication Type:Journal Article
- Keywords:
the next generation sequencing;
endometrial carcinomas;
molecular classification;
clinical application
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2023;44(2):217-223
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the clinical application of molecular classification in endometrial cancers with the next generation sequencing （NGS）. MethodsTotally 112 cases of endometrial carcinoma diagnosed by pathology in The Sun Yat-sen University Cancer Center were collected. All of them were tested by hybridized-capture second-generation sequencing based on 1，021 gene panel. The molecular variation spectrum of each subtype and its relationship between the clinicopathological features were analyzed. ResultsThe cases were distributed as follows： 8 （7.1%） POLE mutation， 34 （30.4%） mismatch repair deficient， 26 （23.2%） TP53 mutation， 44 （39.3%） non-specific molecular profile. The median tumor mutation burden was respectively 252.0， 38.4， 5.8 and 5.4 Muts/Mb. There were no significantly differences among four subtypes in clinicopathological features such as age， histological grade， lymph node metastasis and clinical stage. PTEN （75.5%）， PIK3CA （66.7%）， ARID1A （55.9%）， TP53 （40.2%）， NF1 （29.4%） were the most common mutations in endometrial cancers. ConclusionsThe utilization of NGS in endometrial cancers can simultaneously identify molecular subgroups， screen Lynch syndrome and obtain molecular variation spectrum， which can provide guidance for immunotherapy and targeted therapy， contribute to further accumulation and exploration of molecular genetic characteristics.
