Characterization of a brain tumor cell line established from transgenic mice expressing the vasopressin SV-40 T antigen.
- Author:
Sung Hyun KIM
1
;
Myoung Ok KIM
;
Sang Ryeul LEE
;
Kil Soo KIM
;
Tae Hoon LEE
;
Hoon Taek LEE
;
Ji Hong HA
;
Tae Yoon KIM
;
Zae Young RYOO
Author Information
1. School of Life Science and Biotechnology, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea. jaewoong64@hanmail.net
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
astrocyte cell;
brain tumor cell;
neuronal cell;
transgenic mice;
vasopressin
- MeSH:
Vasopressins/genetics/*metabolism;
Transgenes/genetics;
Recombinant Fusion Proteins/genetics/metabolism;
Plasmids/genetics;
Mice, Transgenic;
Mice, Inbred ICR;
Mice;
In Situ Hybridization, Fluorescence/methods;
Immunoenzyme Techniques;
Gene Expression/genetics;
Cell Proliferation;
Cell Line, Tumor;
Brain Neoplasms/genetics/*metabolism/pathology;
Blotting, Western;
Antigens, Polyomavirus Transforming/genetics/*metabolism;
Animals
- From:Experimental & Molecular Medicine
2006;38(3):196-202
- CountryRepublic of Korea
- Language:English
-
Abstract:
We previously reported that transgenic mice produced with a transgene consisting of the SV40 T antigen and vasopressin without the 3'-flanking region exhibit brain tumors and lymphoma. In this study, transgenic mice were produced with the fusion gene containing the SV40 T antigen and the whole vasopressin gene with the 3'-flanking region. Six transgenic mice were generated, five which died after 2-6 weeks. The remaining founder mouse was investigated for fusion gene expression and tumor progression at the age of 6 weeks. Brain tumor cells were characterized for phenotypes and transgene expression. During in vitro cell cultures, the phenotypic appearances at 10, 20, and 30 passages were as a uniform monolayer with similar growth rates. The site of SV40 T antigen integration was in the A2 region of chromosome 11, and SV40 T antigen was expressed at the same level in cells of both earlier and later passages. Thirty passages were probably insufficient to reach crisis and immortalization. These cells enriched brain tumor cell compositions with astrocytes and neuronal cells.
