Value of chromosomal microarray analysis for genetic evaluation of fetal ultrasound abnormality
10.13491/j.issn.1004-714X.2022.05.017
- VernacularTitle:染色体微阵列技术用于超声结构异常胎儿遗传学评估的价值
- Author:
Linling XIAO
1
;
Jun XU
1
;
Xiaohong ZHANG
1
;
Guilan GUO
1
;
Jufang TAN
2
;
Li HE
1
;
Shuang ZHANG
1
Author Information
1. Center for Ultrasound Medicine, Chenzhou First People’s Hospital, Chenzhou 423000 China.
2. Center for Prenatal Diagnosis, ChenzhouFirst People’s Hospital, Chenzhou 423000 China.
- Publication Type:OriginalArticles
- Keywords:
Chromosomal microarray analysis;
Genetic diagnosis;
Ultrasound abnormality;
Copy number variation
- From:
Chinese Journal of Radiological Health
2022;31(5):611-614
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the value of chromosomal microarray analysis (CMA) for genetic evaluation of fetal ultrasound abnormality. Methods A total of 180 pregnant women with fetal abnormality detected by prenatal ultrasound diagnosis in the first trimester during the period from January 2020 through May 2022 were enrolled as the study subjects. All prenatal fetal screening samples were subjected to G-band karyotyping and CMA. Results G-band karyotyping detected normal karyotypes in 168 samples (93.85%) and abnormal karyotypes in 11 samples (6.15%), and CMA detected 17 positive samples (9.44%) and 163 negative samples (90.56%). The seventeen positive samples included 11 pathogenic copy number variations (CNVs) and 6 variants of unknown significance (VOUS), and there were 11 CMA-positive results consistent with G-band karyotyping, and 6 additional pathogenic CNVs mainly included microdeletion and microduplication syndromes. The detection rates of pathogenic CNVs were 11.11%, 2.63%, 2.78%, 4.00%, 0, 0, 11.11% and 0 among the fetuses with abnormal structure of the cardiovascular system, the lymphatic system, the nervous system, the digestive system, the cranial and face system, the skeletal system, the urinary system, and other system (χ2 =8.188, P = 0.316). All eleven fetuses with pathogenic CNVs detected by CMA were all induced for abortion. Conclusion CMA improves the detection of genetic abnormality among fetuses with ultrasound abnormality in relative to G-band karyotyping, which is feasible for prenatal cytogenetic diagnosis among fetuses with ultrasound abnormality
