The target of celastrol acting on HSP60 against pulmonary fibrosis
10.16438/j.0513-4870.2022-0983
- VernacularTitle:雷公藤红素作用于HSP60抗肺纤维化的靶点研究
- Author:
Yu ZHOU
1
;
Ya-zi WEI
1
;
Tian-ming YANG
2
;
Tian-tai ZHANG
1
Author Information
1. State Key Laboratory of Bioactive Substance and Function of Nature Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
2. Tianjin Key Laboratory of Molecular Design and Drug Discovery, Tianjin Institute of Pharmaceutical Research, Tianjin 300301, China
- Publication Type:Research Article
- Keywords:
celastrol;
fibroblast;
pulmonary fibrosis;
heat shock protein 60;
autophagy
- From:
Acta Pharmaceutica Sinica
2023;58(3):688-694
- CountryChina
- Language:Chinese
-
Abstract:
Celastrol, extracted from Tripterygium wilfordii, is a natural pentacyclic triterpene compound, which has an anti-pulmonary fibrosis effect. However, its effect, binding targets and regulatory mechanism in pulmonary fibroblasts remain unclear. In this study, we found that celastrol could prevent fibroblast-myofibroblast transformation (FMT) by significantly inhibiting transforming growth factor β1 (TGFβ1)-induced α-smooth muscle actin and type I collagen expression. Previous studies suggested that heat shock protein 60 (HSP60) may be the target of celastrol. This study confirmed the direct interaction between celastrol and HSP60 through cellular thermal shift assay and surface plasmon resonance experiment, and demonstrated that the KD value of celastrol binding to HSP60 was 8.59 μmol·L-1. Further studies showed that knockdown of HSP60 promoted TGFβ1-induced FMT, especially in the medium and low dose TGFβ1 treatment group, and that the anti-FMT effect of celastrol was significantly weakened after HSP60 knockdown. These results indicated that HSP60 was involved in maintaining the resting state of fibroblasts, and the anti-FMT effect of celastrol was dependent on HSP60. Furthermore, the autophagy promotion and antioxidant effects of celastrol were also weakened after HSP60 knockdown. In conclusion, celastrol inhibits FMT by targeting HSP60, thus exerting anti-pulmonary fibrosis function.
