Monitoring of residual cisatracurium besylate in elderly patients plasma at post operation by UPLC-MS/MS
10.12206/j.issn.2097-2024.202206023
- VernacularTitle:基于UPLC-MS/MS技术监测老年患者术后血浆中苯磺酸顺阿曲库铵残留
- Author:
Jiaming XU
1
,
2
;
Xiaofang ZHOU
2
;
Jianrong GUO
1
,
2
Author Information
1. Postgraduate Training Base of Shanghai Gongli Hospital, Ningxia Medical University, Shanghai 200135, China
2. Department of Anesthesiology, Gongli Hospital, Naval Medical University, Shanghai 200135, China.
- Keywords:
UPLC-MS/MS;
cisatracurium besylate;
content determination;
residual curarization
- From:
Journal of Pharmaceutical Practice
2023;41(3):192-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of cisatracurium besylate in human plasma by UPLC-MS/MS which could be used in the monitoring of drug residual in plasma of elderly patients after operation. Methods The samples were precipitated with 0.1% formic acid-acetonitrile solution and separated by an SHISEIDO ADME column(3.0 mm×100 mm, 2.7 μm) for isocratic elution with the mobile phase of water containing 0.1% formic acid with 2 mmol/L ammonium acetate and acetonitrile (30:70, V/V). MS condition was optimized in the positive ion detection mode by multiple reaction monitoring (MRM), along with the Agilent jet stream electrospray source interface (AJS-ESI). The precursors to the product ion transitions were m/z 464.3→358.2 for cisatracurium besylate and m/z 557.4→356.3 for vecuronium bromide (the internal standard, IS). Plasma samples of elderly patients undergoing spinal surgery were collected after anesthesia induction, at the end of surgery, 0.5 h and 1 h after surgery, and from the blood bags while autologous blood transfusion, and stored in cryopreservation tubes with 2% formic acid solution. Then the contents of cisatracurium besylate were determined. The effects of autogenous blood transfusion on plasma concentration of cisatracurium besylate in elderly patients after surgery evaluated. Results The calibration curve was linear in the range of 20-5 000 ng/ml for cisatracurium besylate in human plasma, r=0.999 7. The intra-day and inter-day precision and accuracy were good (RSD<10%, RE<±10%). The matrix effect of different concentrations was 71.88%~80.64%. The recovery of different concentrations was 83.62%~88.87%. The recovery of vecuronium bromide (IS) was 125.91%, which conformed with the requirement of methodological validation. There was a certain degree of residual cisatracurium besylate in the plasma of elderly patients, so the extubation time should be strictly controlled and the stay time of patients in the anesthesia recovery room should be appropriately extended. Conclusion The method is sensitive, accurate, and efficient, which could be used for the determination of cisatracurium besylate in human plasma of elderly patients after operation.
