Evaluation of toxin-induced injury and protective effect of triptolide based on a cardiovascular chip model
10.12206/j.issn.2097-2024.202206018
- VernacularTitle:基于心血管芯片模型的4种深海毒素致伤评价与雷公藤甲素的保护作用研究
- Author:
Xiaoli HE
1
,
2
;
Yiwei SHI
2
;
Lan CHEN
1
,
2
;
Yue LIU
2
;
Zhanying HONG
1
,
2
Author Information
1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
2. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Keywords:
cardiovascular chip;
marine biotoxins;
glycocalyx damage;
triptolide
- From:
Journal of Pharmaceutical Practice
2023;41(3):149-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a cardiovascular chip model for evaluating the damage of vascular glycocalyx induced by four marine toxins: okadaic acid (OA), conotoxin (CTX), tetrodotoxin (TTX) and gymnodimine (GYM), and explore the protective effect of triptolide on toxin-induced injury. Methods Human umbilical vein endothelial cells（HUVEC) were inoculated into a three-channel microfluidic chip. CCK-8 method and immunofluorescence staining were used to analyze the damage of cell viability and glycocalyx tissue induced by low, middle and high concentrations of marine toxin, as well as the protective effect of triptolide on toxin-induced injury. Results The cells in the cardiovascular chip grew well and had structurally intact glycocalyx. Compared with the control group, the activity of HUVEC cells were inhibited in group of the medium and high concentration of OA and high concentration of GYM (P<0.05). The activity of cells had not been inhibited by CTX and TTX significantly , but all the four toxins caused serious damage to the glycocalyx tissue (P<0.01). After pre-protection with triptolide, the toxicity of the four toxins to HUVEC cells and the damage rate of glycocalyx decreased significantly. Conclusion The four marine biotoxins could damage the activity and glycocalyx of HUVEC cells in a dose-dependent manner, while triptolide has a protective effect on HUVEC cells injured by toxin.
