Thermal stability of neuraminidase in influenza vaccine
- VernacularTitle:流感疫苗中神经氨酸酶的温度稳定性分析
- Author:
CAO Hai dan
1
;
QIU Lu
;
ZHAO Li jia
;
XU Wen
;
WANG Min
;
LI Wen qian
;
LI Shuai
Author Information
- Publication Type:Journal Article
- Keywords: Influenza vaccine；Neuraminidase（NA）；Temperature stability
- From: Chinese Journal of Biologicals 2023;36(1):16-20
- CountryChina
- Language:Chinese
- Abstract: Abstract：Objective To analyze the stabilities of neuraminidase（NA）in influenza vaccine at different temperatures and provide a reference for further complete understanding of overall shelf life of vaccines. Methods Monovalent bulks of influenza H1N1，H3N2 and B vaccines were stored at 4（low temperature），25（room temperature）and 37 ℃（changed temperature）for 0. 5，2，7，24 and 48 h separately，using that at 100 ℃（extreme temperature）for 1 h as control，and determined for NA activity by enzyme⁃linked lectin method. Results The NA activities of influenza H1N1 vaccines stored at 25 and 37 ℃ decreased significantly with the increasing of time. No significant decreases were observed in H3N2 and B vaccines even after storage at two non⁃storage temperatures for 48 h. However，all the NA activities of three vaccines decreased at 100 ℃. Conclusion Both H3N2 and B vaccines showed high stability at abnormal storage temperatures not more than 37 ℃，while H1N1 vaccine was relatively sensitive to the temperature for storage.
- Full text:流感疫苗中神经氨酸酶的.pdf
