Evaluation of inhibitory effect of tumor vaccine in colon carcinoma model mice
10.13200/j.cnki.cjb.003800
- VernacularTitle:肿瘤疫苗在结肠癌模型小鼠体内抑制效果的评价
- Author:
Lu HAN
1
;
LIANG Zhao yuan
;
SHI Si wei
;
YANG Li qun
;
DENG Xiong wei
;
SHENG Wang
Author Information
1. *Faculty of Environment and Life，Beijing University of Technology，Beijing 100124，China
- Publication Type:Journal Article
- Keywords:
Tumor vaccine；Tumor lysate；CpG β⁃glucan nanoparticles（CNP）；Colon carcinoma
- From:
Chinese Journal of Biologicals
2023;36(1):11-15+20
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the inhibitory effect of tumor vaccines in colon carcinoma model mice.
Methods: Mouse bone marrow⁃derived dendritic cells（BMDCs）were stimulated by using CpG β⁃glucan nanoparticles（CNP）in vitro. The
BMDCs were divided into PBS group，NP group（without CpG nanoparticles），Lysate group（MC38 cell lysate）and CpG
group（CpG1826），which were determined for the expression of marker molecules on the surface by flow cytometry and for the
contents of interleukin⁃6（IL⁃6）and IL⁃12p40 in the culture supernatant by ELISA. The tumor lysate nano⁃vaccine was pre⁃
pared by mixing 50 mg／mL tumor lysate（MC38 cell lysate）with 200 mg／mL CNP in a volume ratio of 1∶1，with which
mice were subcutaneously immunized as Vaccine group. Vaccine group，PBS group，CNP group and Lysate group were im⁃
munized once a week，for three times in total. Mice were subcutaneously inoculated with MC38 cells，2 × 105 cells for each，
in the right lower limb 1 h after the last immunization，and measured for tumor volume once every three days to plot the
tumor growth curve. The ratios of CD3+ CD4+ T and CD3+ CD8+ T cells in the blood were analyzed by flow cytometry and the
levels of tumor necrosis factor⁃α（TNF⁃α）and interferon γ（IFNγ）in the blood and spleen of mice were determined by
ELISA.
Results: CNP effectively increased the expression of CD11c+ CD80+，CD11c+ CD86+，CD11c+ MHC⁃Ⅱ+ and the secretion of IL⁃6 and IL⁃12p40 in BMDCs in vitro，which were significantly higher than those in other 4 groups（t = 4. 3 ~
46. 2，each P < 0. 05）. Compared with that of the other three groups，the tumor volume of mice in Vaccine group decreased
significantly（t =2.6~3.4，eachP <0. 05）；TherewasnosignificantdifferenceinCD3+ CD8+ TandCD3+ CD8+ Tcellratios（t =
0.5~ 1. 9，each P > 0. 05）；The content of IFNγ in blood increased significantly（t = 3. 8 ~ 4. 6，P < 0. 05），while thatof
TNF⁃α showed no significant difference（t = 0. 4 ~ 2. 0，each P > 0. 05）；However，the contents of IFN γ and TNF⁃α in
spleen increased significantly（t = 6. 3 ~ 13. 0，each P < 0. 001）.
Conclusion:The prepared nano⁃vaccine of tumor lysate
improvedtheimmune level in mice and effectively inhibited the growth of colon carcinoma.
- Full text:肿瘤疫苗在结肠癌模型小鼠体内抑制效果的评价1.pdf
