Antigen presentation and T cell activation by dendritic cells in radiation damage
10.13491/j.issn.1004-714X.2022.06.003
- VernacularTitle:DC细胞在辐射损伤抗原递呈及T细胞活化中的作用
- Author:
Qian LI
1
;
Shuang GENG
2
;
Chengming YAN
2
;
Haoxin GUO
1
;
Zhixin WANG
2
;
Meiyu WANG
3
,
4
;
Benbo LIU
2
;
Xu WANG
2
;
Yilong WANG
2
;
Zhihua YANG
2
;
Maoxiang ZHU
3
,
4
,
5
Author Information
1. School of Public Health, University of South China, Hengyang 421001 China.
2. Institute of Radiation Medicine, Academy of Military Medicine, Academy of Military Sciences, Beijing Key Laboratory of Radiobiology, Beijing 100850 China.
3. Institute of Radiation Medicine, Academy of Military Medicine, Academy of Military Sciences, Beijing Key Laboratory of Radiobiology, Beijing 100850 China
4. College of Life Sciences, Hebei University, Baoding 071002 China.
5. School of Public Health, University of South China, Hengyang 421001 China
- Publication Type:Journal Article
- Keywords:
Ionizing radiation;
Lung epithelial cells;
Dendritic cells;
T cells;
Antigen presentation
- From:
Chinese Journal of Radiological Health
2022;31(6):657-662
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore dendritic cells (DCs)-mediated antigen presentation for radiation-injured cells by using the in vitro cell co-culture technology to simulate the in vivo microenvironment of the lung tissue. Methods 60Co γ-irradiated mouse lung epithelial cells (MLE-12) were cultured with bone marrow-derived DCs and/or splenic T lymphocytes for 48 hours. Flow cytometry was used to measure the expression levels of costimulatory molecules (CD80/86) and antigenic peptide recognition complexes (the major histocompatibility complex [MHC] class Ⅰ/Ⅱ) on DCs and T cell activation markers (CD69/28/152) as well as the numbers of CD4+ and CD8+ T cells. Results 60Co γ irradiation significantly increased the apoptosis rate of MLE-12 cells in a dose-dependent manner, and significantly stimulated the expression of CD80/86 and MHC Ⅱ on DCs, without direct activation of T cells. After γ (6 Gy)-irradiated MLE-12 cells were co-cultured with DCs and T lymphocytes for 48 h, there were significant increases in the expression of CD69 and CD28 on T cells, the numbers of CD4+ and CD8+ T cells, and the expression of CD86 and MHC I on DCs, as compared with the control groups. Conclusion Radiation-injured cells can stimulate antigen presentation by DCs and activate T cells.
