Application of CRISPR/Cas systems in the nucleic acid detection of pathogens: a review
10.16250/j.32.1374.2022227
- VernacularTitle:CRISPR/Cas系统在病原体核酸检测中的应用进展
- Author:
Mengru LI
1
;
Zhiqiang QIN
1
;
Kun YIN
2
;
Bin ZHENG
1
Author Information
1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, National Health Commission Key Laboratory on Parasite and Vector Biology, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, School of Global Health, Chinese Center for Tropical Diseases, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
2. Shanghai Jiao Tong University School of Medicine, China
- Publication Type:Journal Article
- Keywords:
CRISPR/Cas system;
Nucleic acid detection;
Virus;
Bacterium;
Parasite
- From:
Chinese Journal of Schistosomiasis Control
2023;35(1):98-103
- CountryChina
- Language:Chinese
-
Abstract:
Rapid, sensitive and specific detection tools are critical for the prevention and control of infectious diseases. The in vitro nucleic acid amplification assays, including polymerase chain reaction and isothermal amplification technology, have been widely used for the detection of pathogens. Recently, nucleic acid detection-based on clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) have been developed, which are rapid, highly sensitive, highly specific, and portable. This review describes the classification and principle of CRISPR/Cas systems and their applications in pathogen detection, and discusses the prospects of CRISPR/Cas systems.
