Migration and Survival of Rat Neural Stem Cells Marked by Green Fluorescent Protein after Transplanted to Injured Spinal Cord
- VernacularTitle:绿色荧光蛋白标记的大鼠神经干细胞移植于损伤脊髓后的迁移和存活
- Author:
Chuanchuan GAO
;
Lei XIA
;
Shuyu HAO
;
Junhua LI
;
Hong WAN
- Publication Type:Journal Article
- Keywords:
green fluorescent protein, lentiviral vectors, neural stem cells
- From:
Chinese Journal of Rehabilitation Theory and Practice
2008;14(4):341-342
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the migration and survival of neural stem cells(NSCs)in vivo.Methods NSCs cultured in vitro were transfected by lentiviral vectors expressing green fluorescent protein(GFP)to construct GFP-NSCs,then trans-seeded into lactide-co-glycolide(PLGA)scaffold and implanted into the injured site of T9 spinal cord in rat.One month after transplantation,the migration of NSCs in spinal cord was examined by fluorescence microscope,and the survival rate of NSCs was counted out.Results NSCs labeled GFP had strong expression of green fluorescence.One month after transplanting,part of NSCs expressing GFP could be seen in PLGA scaffolds and rostral,caudal spinal core.The survival rate counted out was 1.4911±0.0313%.Conclusion NSCs marked by GFP and transplanted to rat injured spinal cord could migrate into the spinal cord tissues and the minority of them could survive.
