Effects of Tetrahydroxy-stilbene Glycoside on Over-expression of α-synuclein Protein in Transgenic COS-7 Cells
- VernacularTitle:二苯乙烯苷对过度表达α-synuclein蛋白的转基因COS-7细胞株的影响
- Author:
Ying LIU
;
Lin LI
;
Hui YANG
;
Yali LI
;
Lan ZHANG
- Publication Type:Journal Article
- Keywords:
4'-tetrahydroxy stilbene-2-β-D-glycoside(TSG), Polygonum multiflorum, α-synuclein, COS-7 cell, in vitro
- From:
Chinese Journal of Rehabilitation Theory and Practice
2008;14(4):338-340
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of 2,3,5,4'-tetrahydroxy stilbene-2-β-D-glycoside(TSG)on the over-expression and aggregation of α-synuclein in vitro.Methods TSG in different concentrations was incubated with α-synuclein transgenic COS-7 cells for 24 h.The cell viability was measured by MTT method.The expression of α-synuclein protein was determined by immunocytochemistry and Western blotting method.Results Incubation of TSG at the range of 12.5～200.0 μmol/L with α-synuclein transgenic COS-7 cells for 24 h did not influence cell viability,but a dose-dependently inhibition for the over-expression of α-synuclein protein could be observed in the tests of immunocytochemistry and Western blotting.Conclusion TSG can inhibit the over-expression of α-synuclein protein in COS-7 cells in vitro.
