Analysis on Quasispecies of HIV-1 env Gene by Single Clone per PCR.
- Author:
Han Na YANG
1
;
Jeong Gu NAM
;
Sung Soon KIM
;
Sang Yun CHOI
;
Joo Shil LEE
Author Information
1. Center for AIDS Research, Department of Virology, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Korea. jooshil@nih.go.kr
- Publication Type:Original Article
- Keywords:
HIV;
env gene;
Quasispecies;
Molecular cloning
- MeSH:
Clone Cells*;
Cloning, Molecular;
Genes, env*;
Genetic Variation;
HIV;
HIV-1*;
Polymerase Chain Reaction*;
RNA-Directed DNA Polymerase;
Tropism
- From:Journal of Bacteriology and Virology
2005;35(2):133-140
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The human immunodeficiency viruses (HIV) exhibit tremendous genetic variability in their hosts. It is mainly due to two factors: the error-prone nature of the viral reverse transcriptase enzyme and the effects of environmental constraints such as antiviral therapy, cellular tropism, or HIV-specific immune responses. These quasispecies show fluctuation both in the overall divergence and diversity between individual sequences with different duration after primary infection. For better understand the viral quasispecies, we have performed the longitudinal genetic analysis of HIV-1 env gene V1-C5 region (1.2 kb) by two molecular cloning methods. Diversity indicated that 'single clone per PCR' value was higher than that of 'multiple clones per PCR' in subjects: 0.58-3.15 in subject 1 (P<0.05) and 0.28-2.25 in subject 2 (P<0.05). But divergence was similar in both molecular cloning methods. Phylogenetic analysis of longitudinal sequences at different sampling stages revealed the existence of different topologies individually. These data suggested that 'single clone per PCR' is more efficient than 'multiple clones per PCR' in genetic diversity analysis.
