Mechanism of M2 macrophage-derived exosomes in promoting the migration of glioblastoma via transferring miR-1260b
10.11665/j.issn.1000-5048.2023020901
- VernacularTitle:M2巨噬细胞来源的外泌体转运miR-1260b促进胶质母细胞瘤迁移的机制研究
- Author:
Heng ZHANG
1
;
Qianqian CHEN
;
Yanping GUI
;
Min HUANG
;
Yabing GUO
;
Li ZHAO
Author Information
1. 中国药科大学基础医学与临床药学学院
- Publication Type:Journal Article
- Keywords:
glioblastoma;
M2 macrophage;
exosomes;
miR-1260b;
AJAP1
- From:
Journal of China Pharmaceutical University
2023;54(1):95-106
- CountryChina
- Language:Chinese
-
Abstract:
Tumor-associated macrophage promotes the progression of glioblastoma (GBM) by infiltrating into tumor tissue, yet its mechanism has not been fully elucidated.This paper aimed to investigate the mechanism of M2 macrophages in affecting the migratory capacity of GBM via secreting exosomes.Ultracentrifugation was used to extract exosomes; RNA sequencing was carried out to screen differentially expressed miRNAs; target prediction database was used to predict the possible target proteins of miRNA; Dual-luciferase reporter assay was performed to verify the interaction between miRNA and target genes; and the proliferation ability of tumor cells was detected by subcutaneous xenograft model in nude mice.Results showed that tumor-related macrophages were mainly M2 macrophages, and that exosomes secreted by M2 macrophages could promote the migration of glioma cells.Meanwhile, exosomes secreted by M2 macrophages transported miR-1260b and affected the migration of glioma cells through directly targeted AJAP1, suggesting that exosomes secreted by macrophages could affect the migration ability of GBM through transporting miR-1260b.
