Acellular Organismic Scaffolds for Small-diameter Vascular Tissue Engineering
- VernacularTitle:小口径组织工程血管脱细胞生物支架材料的研究
- Author:
Guofeng LIU
;
Daping YANG
;
Tiefang GUO
;
Chenguang HAO
;
Chunlei NIE
;
Zhijuan HE
- Publication Type:Journal Article
- Keywords:
tissue engineering, vascular graft, scaffold, extracellular matrix, decellularization
- From:
Chinese Journal of Rehabilitation Theory and Practice
2008;14(3):234-236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the acellular carotid artery of adult pig using biotic enzymes for small-diameter vascular tissue-engineering scaffold.Methods Carotid artery of adult pig was removed cells by using Trypsin/EDTA,ribonuclease and desoxyribonuclease.Residual cellular composition was studied with quantitative DNA analysis and the Hematoxylin and Eosin(H&E)staining.Extracellular matrix composition was evaluated with elastin van Gieson's(VG)staining and scanning electron microscopy(SEM).Results Quantitative DNA analysis and H&E staining confirmed that carotid arteries were completely decellularized.Elastin van Gieson's staining demonstrated that elastin morphology appeared unchanged.Scanning electron microscopy examination of the acellular scaffolds revealed a well-oriented porous decellularized structure that maintained natural architecture of the aorta.Conclusion Carotid artery of adult pig rendered acellular with Trypsin/EDTA,ribonuclease and desoxyribonuclease has well-preserved extracellular matrix for vascular scaffold,which can be used as a scaffold for further small-diameter vascular tissue engineering.
