Heterogeneous Composition of Voltage-Dependent K+ Currents in Hepatic Stellate Cells.
10.3349/ymj.2007.48.4.684
- Author:
Dong Hyeon LEE
1
;
Kuchan KIMM
;
Hyung Lae KIM
;
Bok Ghee HAN
Author Information
1. Division of Biobank for Health Sciences, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul, Korea. bokghee@nih.go.kr
- Publication Type:Original Article
- Keywords:
Hepatic stellate cells;
voltage-dependent K(+) currents;
different cell population
- MeSH:
Animals;
Cells, Cultured;
Electric Conductivity/classification;
Hepatocytes/*chemistry/classification;
Ion Transport;
Patch-Clamp Techniques;
Potassium Channels, Voltage-Gated/*physiology;
Rats
- From:Yonsei Medical Journal
2007;48(4):684-693
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Hepatic stellate cells (HSC) are a type of pericyte with varying characteristics according to their location. However, the electrophysiological properties of HSC are not completely understood. Therefore, this study investigated the difference in the voltage-dependent K(+) currents in HSC. MATERIALS AND METHODS: The voltage-dependent K(+) currents in rat HSC were evaluated using the whole cell configuration of the patch-clamp technique. RESULTS: Four different types of voltage-dependent K(+) currents in HSC were identified based on the outward and inward K(+) currents. Type D had the dominant delayed rectifier K(+) current, and type A had the dominant transient outward K(+) current. Type I had an inwardly rectifying K(+) current, whereas the non-type I did not. TEA (5mM) and 4-AP (2mM) suppressed the outward K(+) currents differentially in type D and A. Changing the holding potential from -80 to -40mV reduced the amplitude of the transient outward K(+) currents in type A. The inwardly rectifying K(+) currents either declined markedly or were sustained in type I during the hyperpolarizing step pulses from -120 to -150mV. CONCLUSION: There are four different configurations of voltage-dependent K(+) currents expressed in cultured HSC. These results are expected to provide information that will help determine the properties of the K(+) currents in HSC as well as the different type HSC populations.
