Secondary metabolites of the endophytic fungus Aspergillus sp.Dq-25 from barnacle
10.11665/j.issn.1000-5048.20220531001
- VernacularTitle:一株藤壶内生真菌的次生代谢产物
- Author:
Caihong CAI
1
;
Hao ZHENG
;
Cuijuan GAI
;
Haofu DAI
;
Wenli MEI
;
Huiqin CHEN
Author Information
1. 中国热带农业科学院热带生物技术研究所
- Publication Type:Journal Article
- Keywords:
barnacle;
endophytic fungus;
Aspergillus sp.;
γ-butyrolactone;
chemical constituents;
isolation and identification;
cytotoxicity
- From:
Journal of China Pharmaceutical University
2023;54(1):62-67
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents of solid rice culture of the endophytic fungus Aspergillus sp.Dq-25 from barnacle were isolated and purified by silica gel, Sephadex LH-20, C18 reversed silica gel column chromatography and recrystallization.Their structures were identified by the physical and chemical properties, and by various spectroscopic methods.Six compounds were isolated and identified as: demethyldihydropenicillic acid (1), dihydropenicillic acid (2), penicillic acid (3), fortisterol (4), 22E, 24R-3P, 5a-dihydroxyerogosta-7, 22-diene-6-one (5), and (22E, 24R)-ergosterol-7, 22-diene-3β, 5α, 9α-triol-6-one (6).Compound 1 was a new butyrolactone.MTT method was used to analyze cytotoxicity, and the result showed that compound 3 exhibited inhibitory activity on five cell lines, including K562, HeLa, SGC-7901, A542 and BEL-7402, with IC50 values of 38.0 ~ 105.0 μmol/L.
