The change of gene expression in chrysotile asbestos-induced malignanttransformation of bronchial epithelial cells
- Author:
LIJun xian HUANGHe fei LEIYu shan ZHOUShu yu YUANXiu yuan GAOYa nan ZHULi jin ZHANGFang fang
- Publication Type:Journal Article
- Keywords:
Chrysotile;
Malignanttransformation;
Celltransformation;
Differentiallyexpressedgenes;
Geneexpression;
Signaling
- From:
China Occupational Medicine
2022;51(03):265-
- CountryChina
- Language:Chinese
-
Abstract:
Abstract:Objective -
ToinvestigatetheeffectofchrysotileasbestosongeneexpressioninhumanbronchialepithelialBEAS 2B
Methods -
cells. BEAS 2B cells were randomly divided into two groups. The cells in the chrysotile malignant transformation-
groupweretreatedwith 20μg/cm²chrysotiletoestablishthechrysotileinducedmalignanttransformationBEAS 2Bcellmodel,
andthecellsinthecontrolgroupweretreatedwiththesamevolumeofphosphatesaltbuffersolution.ThetotalRNAinthecells--wasextractedandthecDNAwassynthesizedbyreversetranscription.Cy5dCTPandCy3dCTPfluoresceinwereusedtolabel
the two groups to prepare probes for chip scanning. LuxScan 3.0 image analysis software was used to analyze the fluorescence
signal of labeled DNA, and the differentially expressed genes were screened. The Kyoto Encyclopedia of Genes and Genomes
Results
(KEGG) signaling pathway analysis and Gene Ontology (GO) enrichment analysis were carried out. There were 642
- -
differentiallyexpressedgenes(193up regulatedand449down regulated)inchrysotilemalignanttransformationgroupcompared
with the control group. The KEGG signaling pathway analysis and GO enrichment analysis showed that the differentially
-
expressed genes in the malignant transformed BEAS 2B cells induced by chrysotile asbestos were mainly involved in P53
-
signaling pathway, histone H3 K9 methylation and methylenetetrahydrofolate reductase deficiency pathway, phosphoinositide
binding protein 3 activated protein kinase B signaling pathway, nucleoside phosphate metabolism process and the expression
Conclusion
inhibitionofhistocompatibilitycomplexⅡantigenpresentation. Chrysotileasbestoscaninducethechangeofgene
- -
expression profile in BEAS 2B cells. The P53 signaling pathway, histone H3 K9 methylation and other related pathways are
