Screening and verification of differentially expressed miRNAs in PBMCs of patients with silicosis
- Author:
ZHAOYing zheng CHANGMei yu LIHai bin GUANYi LINing
- Publication Type:Journal Article
- Keywords:
Silicosis Peripheralblood PBMCs Differentialexpression miRNAs Transcriptionfactor Screening Validation
- From:
China Occupational Medicine
2022;51(03):248-
- CountryChina
- Language:Chinese
-
Abstract:
Abstract: Objective
To screen and validate the differentially expressed microRNAs (miRNAs) in peripheral blood
Methods -
mononuclearcells(PBMCs)ofpatientswithsilicosis. Forty eightpatientswithoccupationalsilicosisatstageⅠ(case
group)and45healthycontrols(controlgroup)wereselectedasresearchsubjectsbyrandomnumbertablemethod.PBMCswere-separatedbyFicollPaquegradientcentrifugationfromperipheralblood.Threepeoplefromeachgroupwererandomlyselected
for miRNAs transcriptome sequencing. R Studio software was used to screen differentially expressed miRNAs, and FunRich
software was used to predict the upstream transcription factors related to the differentially expressed miRNAs in PBMCs. The
- Results
differentially expressed miRNAs were verified by real time quantitative polymerase chain reaction. A total of 124
- -
differentiallyexpressedmiRNAswerescreened,amongthem,97miRNAswereupregulatedand27miRNAswere down regulated.-
The 67 targetgenes predicted by differentialmiRNAs were mainly involved in intracellularprocesses and nucleic acid binding
transcriptionfactoractivities,includingcelladhesionmolecules,ratsarcoma,osteoclastdifferentiationandotherpathways.The-------------topfivemiRNAsdifferentiallyupregulatedwerehsamiR1373p,hsamiR500b3p,hsamiR190a5p,hsamiR1413pand------------hsamiR223p.ThetopfivedifferentiallydownregulatedmiRNAswerehsamiR2025p,hsamiR548ai,hsamiR55873p,------hsamiR5705pandhsamiR103983p.ThechangeofthesemiRNAswereconsistentwiththeresultspredictedaccordingto
the miRNAs transcriptome sequencing. The transcription factors including specificity protein 1, early growth response 1, zinc
Conclusion
finger protein 161, etc., were obtained according to the differentially expressed miRNAs. The differentially
