Determination of dimethyl sulfate in workplace air by pre-column derivatization-high performance liquid chromatography
- Author:
YUAN Jing RONG Wei feng HU Jia wen HE Jia heng MENG Rui bo WU Chuan WU Bang hua
- Publication Type:Journal Article
- Keywords:
Workplace;
Air;
Dimethyl sulfate;
Silicone tube;
Precolumn derivatization;
High performance liquid chromatography Determination
- From:
China Occupational Medicine
2022;52(04):425-
- CountryChina
- Language:Chinese
-
Abstract:
Abstract: Objective - -
To establish a pre column derivatization high performance liquid chromatography method for detecting
Methods
dimethyl sulfate (DMS) in workplace air. DMS in workplace air was collected with mercaptopyridine impregnated
（
silicone tube. The derivative of DMS and mercaptopyridine was eluted by mobile phase phase A: water, phase B: acetonitrile,
∶
the volume ratio was 40 60) , and separated with a C18 column, then detected with diode array detector and quantitated by a
Results -
standard curve. The linear range of DMS was 0.17 40.00 mg/L, with the correlation coefficient of 0.999 95. The
detection limit and the lower limit of quantitation were 0.05 and 0.17 mg/L respectively. The minimum detection concentration
and minimum quantitation concentration were 0.02 and 0.04 mg/m³, respectively (air sample volume of 4.5 L, 1.0 mL sample
- - -
solution). The average desorption efficiency was 98.40% 102.00%. The within run and between run relative standard deviations
- -
were 0.61% 3.92% and 1.71% 6.00%, respectively. The samples could be stored at room temperature for at least 14 days.
Conclusion
This method can be used to detect DMS in workplace air.
