Analyzing the difference between atomic fluorescence spectrometry and ICP-MS in the determination of arsenic in urine
- Author:
LIANG Jia bin HE Yi nan GAO Yun xia GUO Jia ming GUO Yao ping CHEN Jiu LIU Yi min WANG Zhi
- Publication Type:Journal Article
- Keywords:
Atomic fluorescence spectrometry ICP MS Urine Arsenic Inorganic arsenic Arsenic betaine Arsenic choline
- From:
China Occupational Medicine
2022;52(04):421-
- CountryChina
- Language:Chinese
-
Abstract:
Abstract: Objective （ ）
To compare the measured results of arsenic in urine by atomic fluorescence spectrometry AFS and
- （ - ）， Methods
inductively coupled plasma mass spectroscopy ICP MS and analyze the reasons of the difference. The samples
WS/T 474-2015 Determination of Arsenic in Urine by Hydride Generation Atomic Fluorescence
were pretreated according to
Spectrometry， （ ∶ ∶ ∶∶ ，V/V/V）
and digested with mixed acid nitric acid sulfuric acid perchloric acid=3 1 1 and then determined by
- -
AFS and ICP MS. The samples were diluted with 0.50% nitric acid and determined by ICP MS. The samples included urine
， ， （
arsenic quality control samples inorganic arsenic supplemented samples and organic arsenic arsenic choline and arsenic
） -
betaine supplemented samples. Standard curve method was used to compare the results of AFS method and ICP MS method.
Results （ ） （ ）
The results of quality control samples by AFS method digestion and ICP-MS method without digestion were
， -
within the range of reference values but the values obtained by AFS method were lower than those obtained by ICP MS method.
- - - ，
The recovery of AFS and ICP MS was 97.79% 100.82% and 99.55% 99.98% respectively. In the middle and high
， - （ P ）
concentration groups the measured values of inorganic arsenic by AFS were lower than that by ICP MS all <0.01 . The
（ ） -
recovery of arsenic betaine and arsenic choline by AFS method digestion was only 2.17% 2.63%. The values of arsenic betaine
（ ） - （
and arsenic choline measured by AFS method digestion were lower than those measured by ICP MS method without
） - （ ）（ P ）Conclusion
digestion and ICP MS method digestion all <0.01 . The result of urine arsenic measured by AFS method
- ，
was lower than that measured by ICP MS method which may be related to the mixed acid digestion of AFS method.
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