Dual Expression of Two Transgenes Introduced by Lentiviral Vectors.
- Author:
Su Jung PARK
1
;
Sun Ju CHOI
;
Joo Young PARK
;
Kyoung Ho LEE
Author Information
1. Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Kangwon-Do, 220-701, Korea. leekh@wonju.yonsei.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
HIV-1;
lentiviral vector;
transgene;
dual expression
- MeSH:
Encephalomyocarditis virus;
Fluorescent Antibody Technique;
HIV-1;
Lentivirus;
Peptide Elongation Factor 1;
Ribosomes;
Transgenes*
- From:Journal of Bacteriology and Virology
2005;35(2):157-164
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Lentiviruses can infect mitotic and non-dividing cells owing to the karyophilic properties of their pre-integrating complex, which allow its active import through the nucleopore. Thus lentiviral vectors derived from human immunodeficiency virus type 1 can mediate an efficient transfer integration, and stable expression of transgenes into proliferating and stationary cells both in vivo and in vitro. By adopting the internal ribosome entry site of encephalomyocarditis virus for bicistronic expression or two promoters of EF-1alpha and SV40 for separate expression of two genes of interest, we developed two lentiviral vectors that express two genes. On FACS analysis, RT-PCR, and immunofluorescence assay, it was shown that the target cells expressed two genes of interest at different levels as the transducing vectors designed for. This vector system is useful especially for a stable, dual-gene expression and two transgene deliveries to non-dividing cells.
