Flow Cytometric Analysis of Lymphocyte Subpopulations of Cattle Infected with Bovine Leukemia Virus.
- Author:
Soon Seek YOON
1
;
You Chan BAE
;
Yi Seok JOO
;
Seong Hwan WEE
;
Kyung Woo LEE
;
Gook Hyun SUH
;
Yong Ho PARK
;
Hong Ryul HAN
Author Information
1. National Veterinary Research and Quarantine Service, Anyang 430-824, Korea. yoonss24@hotmail.com
- Publication Type:Original Article
- Keywords:
bovine leukemia virus;
enzootic;
leukosis;
lymphocyte subpopulation
- MeSH:
Animals;
Antibodies, Monoclonal;
Cattle*;
Enzootic Bovine Leukosis*;
Flow Cytometry;
Immunity, Cellular;
Korea;
Leukemia Virus, Bovine*;
Leukocytes;
Lymphocyte Subsets*;
Lymphocytes*;
Reference Values;
T-Lymphocytes
- From:Journal of Bacteriology and Virology
2005;35(2):165-173
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We examined lymphocyte subpopulations of peripheral blood from BLV infected and noninfected Holstein-Friesian dairy cattle reared in Korea by flow cytometry using monoclonal antibodies specifically reactive with bovine leukocyte differentiation marker. Lymphocyte subpopulations expressing BoCD11b, B-B2, CD5, B, MHC II-DP, MHC II-DQ, and MHC II-DR antigens were significantly abundant in the BLV(+) group than the BLV(-) group (p<0.01). On double staining, subpopulation of B-1a(BoCD5+ BoCD11b+) lymphocytes was significantly increased in leukemic group. However, T-lymphocyte lineage expressing BoCD2, BoCD4, BoCD8, and WC1 antigens was significantly lower than in the BLV(+) group (p<0.01). However the absolute number of T-lymphocytes expressing BoCD2, BoCD4, BoCD8, and WC1 antigens in BLV(+) group remained with in the normal range. Furthermore mean ratio of BoCD4/BoCD8 in the BLV(+) groups was higher than that in the BLV(-) group. Taken together, cellular immune responses did not seem to significantly be decreased in the leukemic cattle.
