Pediococcus pentosaceus as probiotic with cholesterol-lowering ability

Dela Silvia; Lilis Nuraida; Anja Meryandini

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Pediococcus pentosaceus as probiotic with cholesterol-lowering ability

Author: Dela Silvia ¹ ; Lilis Nuraida ^{2
,

3} ; Anja Meryandini ^{4
,

5}
Author Information

1. Postgraduate of Microbiology Study Program, Department of Biology, Faculty of Mathematics and Natural Science, IPB University, 16680, Dramaga, Bogor, Indonesia.
2. Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, IPB University, 16680, Dramaga, Bogor, Indonesia. &
3. Southeast Asian Food and Agricultural Science and Technology (SEAFAST) Center, IPB University, 16680, Dramaga, Bogor, Indonesia
4. Department of Biology, Faculty of Mathematics and Natural Science, IPB University, 16680, Dramaga, Bogor, Indonesia. &
5. Research Center for Bioresources and Biotechnology (RCBIO), IPB University, 16680, Dramaga, Bogor, Indonesia.
Publication Type:Journal Article
Keywords: Pediococcus pentosaceus; Probiotics; Cholesterol-lowering
MeSH: Pediococcus pentosaceus
From:Malaysian Journal of Microbiology 2020;16(6):446-453
CountryMalaysia
Language:English
Abstract: Aims:Hypercholesterolemia which is an elevated blood cholesterol level that considered as a major risk factor for cardiovascular disease, which is the leading cause of death in many countries. Therefore, lowering the cholesterol level is important to prevent the disease. Lactic acid bacteria (LAB) group are often used as probiotics for their healthpromotion which include cholesterol-lowering effect. The purpose of this study was to evaluate the potency of Pediococcus pentosaceus as probiotic that could reduce cholesterol.
Methodology and results:All P. pentosaceus strains were able to survive in acid conditions and in the presence of 0.3% bile salts. These strains had antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Salmonella typhimurium ATCC 14028. The LAB were also sensitive to chloramphenicol and showed autoaggregation and coaggregation ability. Pediococcus pentosaceus E5, E7, and E8 were able to remove cholesterol with the highest activity showed by P. pentosaceus E7 (49.00 ± 2.83%). Dead cells and resting cells of P. pentosaceus E5, E7, and E8 (6-22%) also able to reduce the cholesterol but not as effectively as growing cells. Cholesterol lowering is often associated with bile salt hydrolase (BSH) enzyme activity, however none of the isolates were found BSH positive in this study.
Conclusion, significance and impact of study:The present study suggests that P. pentosaceus E7 has beneficial probiotic properties which can be exploited for probiotic product with cholesterol-lowering effect.
Full text:20.2020my0022.pdf