Comparison of characteristic chromatogram and the contents of multiple indicator components of Morus alba decoction powder and decoction at different decoction time
- VernacularTitle:不同煎煮时间桑白皮汤煮散与汤剂的特征图谱及多指标成分含量比较
- Author:
Jinjin JIANG
1
,
2
;
Lei WU
1
;
Yufeng ZHU
1
;
Si SUN
1
;
Mingming QIU
1
;
Chao LU
1
Author Information
1. Dept. of Pharmacy，the Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine，Nanjing 210029，China
2. First School of Clinical Medicine，Nanjing University of Chinese Medicine，Nanjing 210029，China
- Publication Type:Journal Article
- Keywords:
Morus alba decoction;
material basis;
decoction powder;
decoction;
characteristic chromatogram;
content
- From:
China Pharmacy
2023;34(5):565-568
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To compare characteristic chromatogram and the contents of multiple indicator components of Morus alba decoction powder and decoction at different decoction time， and to provide experimental basis for the development of M. alba decoction. METHODS Taking decoction powder and decoction at different decoction time as subject， HPLC characteristic chromatogram of 2 kinds of samples were established with Similarity Evaluation Software System of TCM Chromatographic Fingerprint （2012 version）， and similarity evaluation was performed. The contents of mulberroside A， geniposide， berberine， baicalin， quercetin and luteolin in decoction powder and decoction were determined by HPLC. The contents of each indicator component and the change of total content were as the evaluation indexes to compare the difference between the two substances during decoction. RESULTS The similarities of characteristic chromatogram of the two substances ranged from 0.943 to 1.000 and 0.975 to 0.998 at different decoction time， respectively. Six indicator components of the decoction powder dissolved faster and had higher contents. The contents of each indicator component in the decoction powder when decocting at 20 minutes was 1.1-1.5 times of the decoction when decocting at 50 min， and the total content in the decoction powder was 1.2 times of the decoction. CONCLUSIONS Compared with decoction， M. alba decoction powder has the advantages of shortening the decoction time and saving traditional Chinese medicine resources. The results of this study lay a research foundation for “Zungu” to develop its preparation.
