Effects of raw and wine-processed Schisandra chinensis on neuro-endocrine-immune network in insomnia mice and its mechanism
- VernacularTitle:生、酒五味子对失眠小鼠神经-内分泌-免疫网络的影响及机制
- Author:
Ruiying WANG
1
;
Dan SU
1
;
Huizhen LI
1
;
Yali LIU
2
;
Genhua ZHU
1
;
Ming YANG
3
;
Zhifu AI
1
;
Tao LUO
4
;
Bing XUE
1
;
Yonggui SONG
1
Author Information
1. Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Brain Diseases with Cognitive Impairment/Key Research Room for Chinese Medicine Efficacy Evaluation （Prevention and Treatment of Mental Disorders Brain Disease） of Jiangxi Traditional Chinese Medicine Administration/Key Research Room for Depression Syndrome Animal Models of Jiangxi Traditional Chinese Medicine Administration，Jiangxi University of Chinese Medicine，Nanchang 330004，China
2. Key Laboratory of Efficacy and Safety Evaluation of Jiangxi Health Commission/Key Laboratory of Efficacy and Quality Evaluation of Anti-inflammatory Traditional Chinese Medicine of Jiangxi Administration of Traditional Chinese Medicine，Nanchang Medical College，Nanchang 330004，China
3. Jiangxi Guxiang Jinyun Comprehensive Health Industry Co.，Ltd.，Nanchang 330029，China
4. Blood Purification Center，the First Affiliated Hospital of Nanchang University，Nanchang 330004，China
- Publication Type:Journal Article
- Keywords:
raw Schisandra chinensis;
wine-processed Schisandra chinensis;
neuro-endocrine-immune network;
insomnia
- From:
China Pharmacy
2023;34(5):525-530
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect of raw and wine-processed Schisandra chinensis on neuro-immune-endocrine network in insomnia mice and its mechanism. METHODS Fifty mice were randomly divided into blank group， model group， diazepam group， raw S. chinensis group and wine-processed S. chinensis group， with 10 mice in each group. Except for blank group， the mice in the other groups were intraperitoneally injected with thyroxine solution to establish mice model of insomnia； at the end of each day’s modeling， the corresponding doses of diazepam，raw and wine-processed S. chinensis were given by gavage. The blank group and model group were given constant volume of normal saline. The general state of the mice was observed and recorded， and the total activity distance and upright times of the mice were detected； the EEG and EMG signals of mice were recorded， and the time ratio of sleep wake time （wake）， non-rapid eye movement （NREM） and rapid eye movement （REM） was analyzed； the contents of neurotransmitters [γ-aminobutyric acid （GABA）， 5-hydroxytryptamine （5-HT）， dopamine （DA）， norepinephrine （NE）， cortisol （CORT）] in brain suprachiasmatic nucleus （SCN） were detected； and the expressions of tumor necrosis factor α （TNF-α） and interleukin 1β （IL-1β） were detected； the mRNA expressions of clock gene Bmal1， circadian clock gene Clock and cycle gene Per2 were all detected. RESULTS Compared with the blank group， the mental state of the model group mice was relatively depressed， the amount of food and water increased， the body mass decreased， the hair was rough and shiny， and the circadian rhythm was irregular； the total activity distance and upright times decreased significantly； the time ratio of wake increased significantly， while the time ratios of REM and NREM decreased significantly； the content of 5- HT in brain SCN decreased significantly， while the content of NE， DA and CORT increased significantly； the fluorescence intensity of IL-1β and TNF-α was significantly increased； the relative expression level of Bmal1 and Clock mRNA was significantly increased， while the relative expression level of Per2 mRNA was significantly decreased （P＜0.05 or P＜0.01）. Compared with the model group， the general state of mice in diazepam group， raw S. chinensis group and wine-processed S. chinensis group was improved obviously， and most of the above index levels were significantly reversed （P＜0.05 or P＜0.01）. CONCLUSIONS Raw and wine-processed S. chinensis have a certain therapeutic effect on insomnia mice， the mechanism of which may be related to the regulation of neuro-endocrine-immune system related biological indicators in insomnia mice.
