Gastric Perfusion of Vitamin D3 with Different Diets Induce the Atherosclerotic Rat Model
- VernacularTitle:不同饲料联合维生素D3建立大鼠动脉粥样硬化模型的比较
- Author:
Yaxin YIN
;
Runmei LIU
;
Hongxia ZHAI
;
Liang LI
;
Jinjin ZHANG
;
Haiwei CHEN
;
Lei WANG
;
Na LI
;
Yunfeng XIA
- Publication Type:Journal Article
- Keywords:
atherosclerosis, Vitamin D3, high fat food
- From:
Chinese Journal of Rehabilitation Theory and Practice
2010;16(12):1134-1136
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the optimun approach to induce the atherosclerotic rat model.MethodsThree methods were used to induce atherosclerotic model, including: simple high fat diet feeding, high fat diet feeding with gastric perfusion of Vitamin D3, and gastric perfusion of Vitamin D3 with common rat food (splashed oil on the normal rat food with yolks and peanuts). Histopathology change of the atherosclerotic plaques in aorta was observed by normal and histopathologic sections and Hematoxylin staining.ResultsThe endarterium of aorta of the rats with simple high fat diet was smooth without atherosclerotic plaques after 2 months. 2 rats with high fat diet feeding and gastric perfusion of Vitamin D3 had formed miliary atherosclerotic plaques. In the rats with vitamin D3 and common rat food, the ring-shape calcified atherosclerotic plaques were found in the all endarterium of aorta.ConclusionGastric perfusion of Vitamin D3 once a week (3 times) combined with common rat diet could induce the atherosclerotic model successfully and decrease the mortality rate.
