Inhibitive Effects of RNA Interference on Caspase-3 Gene Expression
- VernacularTitle:RNA干扰对Caspase-3基因表达的抑制作用
- Author:
Jinguang WANG
;
Xin JING
;
Jianmin ZHANG
;
Mingjian ZHANG
;
Chen FU
- Publication Type:Journal Article
- Keywords:
RNA inference, inhibitive effect, Caspase-3
- From:
Chinese Journal of Rehabilitation Theory and Practice
2009;15(4):327-329
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of RNA interference (RNAi) on Caspase-3 expression.Methods According to the gene sequence and the secondary structure characteristics of Caspase-3, three siRNA （Si-Caspase-3-1, Si-Caspase-3-2 and Si-Caspase-3-3） were designed with 21 basic group, and siRNAs against Caspase-3 gene were synthesized. With the liposome conduct, the synthesized siRNAs were transferred into neuroglia cells. After the cells crept the slices, cells were dyed with fluor Hoechst33258; the apoptotic cells were stained with TUNEL, the normal neuroglia cells were as control, the Caspase-3 protease activity were detected with hemi-quantitative RT-PCR and Western blot.Results The synthesized siRNAs could inhibit the expression of Caspase-3 gene. For Si-Caspase-3-3, the expression inhibition rate of Caspase-3 mRNA was 86.32% in neuroglia cell.Conclusion The synthesized siRNAs can inhibit the expression of Caspase-3 gene at cell level.
