Inducing Human Bone Marrow Mesenchymal Stem Cells to Cardiomyo-Like Cells with Supernatant of Normal or Injured Rat Cardiomyocytes in Vitro
- VernacularTitle:正常与损伤条件下乳鼠原代心肌细胞培养上清液对人骨髓间充质干细胞向心肌样细胞的诱导作用
- Author:
Fangge DENG
;
Lianfeng GUO
;
Yingzhi MA
;
Yulin LI
- Publication Type:Journal Article
- Keywords:
human bone marrow mesenchymal stem cells (hMSCs), cardiomyo-like cells, differentiation, cTnI, Desmin
- From:
Chinese Journal of Rehabilitation Theory and Practice
2009;15(3):228-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether human bone marrow mesenchymal stem cells (hMSCs) could differentiate into cardiomyo-like cells by culturing with supernatant of normal or injured rat cardiomyocytes (CMs) in vitro. Methods hMSCs were cultured with supernatant from normal or injured rat CMs for 27~30 d. The morphologies of induced hMSCs were observed with inverted microscope and the special cardio-markers cTnI and Desmin were identified with immunocytochemisry. Results A few cells cultured with supernatant from normal CMs enlarged and expressed cTnI, but little Desmin. While more cells cultured with supernatant from injured CMs enlarged and expressed cTnI, and parts of them expressed Desmin. The incidence of cTnI or Desmin positive cells were significantly different between these two groups (P<0.01). Conclusion Supernatant from both normal and injured CMs can induce hMSCs into cardio-like cells in vitro, and that from injured CMs is more effectively.
