Morroniside Inhibits Hydrogen Peroxide-induced Calcium Overload and Cytotoxicity in SH-SY5Y Neuroblastoma Cells
- VernacularTitle:莫诺苷抑制过氧化氢诱导的SH-SY5Y神经细胞钙超载和细胞毒
- Author:
Wen WANG
;
Fangling SUN
;
Yi AN
;
Wenting HUANG
;
Houxi AI
;
Li ZHANG
;
Lin LI
- Publication Type:Journal Article
- Keywords:
Morroniside, neuroblastoma cell, SH-SY5Y, oxidative stress, calcium overload, cytotoxicity
- From:
Chinese Journal of Rehabilitation Theory and Practice
2009;15(3):201-202
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of morroniside on hydrogen peroxide (H2O2)-induced calcium overload and cytotoxicity in SH-SY5Y neuroblastoma cells. Methods SH-SY5Y cells were pre-incubated with morroniside 1, 10, or 100 μmol/L for 24 h prior to exposure to H2O2 300~500 μmol/L for 18 h. The cytosolic free calcium concentration and LDH release were determined. Results The H2O2-induced cytosolic free calcium concentration decreased in the cells pre-incubated with morroniside 10 or 100 μmol/L, while the LDH release level decreased in the cells pre-incubated with morroniside 1, 10 or 100 μmol/L, comparison with the cells exposed H2O2 along. Conclusion Morroniside effects neuroprotection against H2O2-induced calcium overload and cytotoxicity in SH-SY5Y cell.
