Identifying 1p LOH in Oligodendroglioma with Denaturing High Performance Liquid Chromatography
- VernacularTitle:变性高效液相色谱检测少突胶质细胞瘤1p杂合性缺失
- Author:
Shouwei LI
;
Fang YUAN
;
Tao JIANG
;
Zhongcheng WANG
- Publication Type:Journal Article
- Keywords:
denaturing high performance liquid chromatography, oligodendroglioma, 1p loss of heterozygosity, prognosis
- From:
Chinese Journal of Rehabilitation Theory and Practice
2009;15(2):120-122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up the clinical examination method for 1p loss of heterozygosity (LOH).Methods Genomic DNA was isolated from tumor tissue and peripheral blood in oligodendroglioma patients. Denaturing high performance liquid chromatography (DHPLC) was used to examine the polymerase chain reaction (PCR) Results . 1p LOH was determined by the microsatellite sites.Results 1p LOH was identified by DHPLC Results .Conclusion DHPLC can clearly identify the 1p LOH with short time in oligodendroglioma patients.
