Study on the molecular mechanism of Cinnamomi Cortex-Rehmanniae Radix in preventing and treating osteoporosis based on multi-directional regulation of inflammatory factors, estrogen and bone homeostasis
10.16438/j.0513-4870.2022-0879
- VernacularTitle:基于炎症因子、雌激素及骨稳态的多向调控研究肉桂-熟地黄防治骨质疏松的分子作用机制
- Author:
Fan PING
1
,
2
;
Lin ZHU
1
,
2
;
Xia SHEN
1
,
2
;
Qi-feng HAN
1
;
Ying LIU
1
,
2
;
Yong-gang YAN
1
,
2
;
Gang ZHANG
1
,
2
;
Liang PENG
1
,
2
;
Yan-yan CHEN
1
Author Information
1. College of Pharmacy, Shaanxi University of Chinese Medicine, Xixian New Area 712046, China
2. Shaanxi Qinling Application Development and Engineering Center of Chinese Herbal Medicine, Xixian New Area 712046, China
- Publication Type:Research Article
- Keywords:
Cinnamomi Cortex-Rehmanniae Radix;
osteoporosis;
Chinese medicine compatibility;
metabonomics;
bone homeostasis
- From:
Acta Pharmaceutica Sinica
2022;57(12):3644-3652
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the molecular mechanism of Cinnamomi Cortex-Rehmanniae Radix (CR) in the prevention and treatment of osteoporosis (OP) was investigated by integrating compatibility analysis of compound, bioinformatics and metabolomics. The rat OP models were established, and the Micro-CT indexes and pathological sections were comprehensively evaluated. The results showed that compared with the model group, the indexes such as bone mineral density (BMD) and bone volume/tissue volume (BV/TV) were significantly increased after CR treatment (P < 0.05), and the bone trabeculae were arranged into mesh. The results of UHPLC-Q-TOF/MS mainly involved amino acid metabolism, lipid metabolism and estrogen metabolism pathways. Integrating bioinformatics and metabolomics analysis, it was finally found that: ① cinnamic acid and ethylcinnamate inhibit inflammatory factors such as TNF, IL-1β, and IL-13, thereby preventing and treating OP; ② multiple active ingredients of CR target ESR2, PPARG, and CYP19A1, GABRA1 and other targets, regulate cAMP synthesis, AMPK signaling pathway and lipid metabolism, thereby regulating estrogen levels to prevent and treat OP; ③ oleic acid, arachic acid, etc. act on AR, VDR and other targets, and regulate HIF-1 signaling pathway and AGE-RAGE signaling pathway, thereby regulating osteoblasts and osteoclasts, and affecting calcium and phosphorus absorption to maintain bone homeostasis. This study clarified the molecular mechanism of CR in preventing and treating OP from the perspective of multi-directional regulation of inflammatory factors, estrogen and bone homeostasis, and provided theoretical basis for the clinical application of CR and the development of compound. This experiment complied with the ethical standards of animal experiments and was approved by the Animal Ethics Committee of Shaanxi University of Chinese Medicine (No. SUCMDL20210309002).
