Determination of azelaic acid and potassium azeloycinate diglycinate in cosmetics by HPLC
10.19428/j.cnki.sjpm.2022.21444
- VernacularTitle:高效液相色谱法同时测定化妆品中壬二酸及其衍生物壬二酰二甘氨酸钾的含量
- Author:
Yong XU
1
;
Jing HAN
1
;
Ling YU
1
;
Taicheng HUI
1
;
Jun HUANG
2
;
Xingsheng PENG
1
;
Rong ZHENG
1
Author Information
1. Shanghai Institute for Food and Drug Control， NMPA Key Laboratory for Monitoring and Evaluation of Cosmetics， Shanghai 201203， China
2. East China University of Science and Technology， Shanghai 200237， China
- Publication Type:Journal Article
- Keywords:
azelaic acid;
potassium azeloycinate diglycinate;
HPLC;
cosmetics
- From:
Shanghai Journal of Preventive Medicine
2022;34(12):1250-1256
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveAn HPLC method was established for the determination of azelaic acid and potassium azeloycinate diglycinate in cosmetics. MethodsThe samples were extracted with 60 mmol·L-1 sodium hydroxide water solution-methyl alcohol. After centrifugation and filtration， the analysis of azelaic acid and potassium azeloycinate diglycinate was performed with a SVEA C8（250 mm×4.6 mm， 5 μm） column， using 15 mmol‧L-1 potassium dihydrogen phosphate solution （pH=3.0） and acetonitrile for gradient elution at a flow rate of 1.0 mL·min-1.The analytes were detected with UV detector， and quantified by external standard curve. ResultsThe results showed a good linearity in the range of 5‒1 000 μg‧mL-1 with correlation coefficients （r） larger than 0.999. The detection limit of azelaic acid and potassium azeloycinate diglycinate （LOD） was 0.020% and 0.015%， respectively. The spiked recoveries were 87.66% to 108.96% with the relative standard deviation （RSD） of 0.6% to 3.3%. ConclusionThe method is simple， rapid and highly sensitive. It is suitable for the determination of azelaic acid and potassium azeloycinate diglycinate in cosmetics.
