A New Rapid Method for Preparing Synaptosomes of Mouse
- VernacularTitle:小鼠脑突触体制备方法的改进
- Author:
Zhongxin XIAO
;
Shougang WEI
;
Shaojun YUN
;
Xiaojuan HE
;
Shuhua ZHANG
;
Qing CAI
;
Qiang LU
;
Man JI
- Publication Type:Journal Article
- Keywords:
Synaptosomes, transmission electron microscopy
- From:
Chinese Journal of Rehabilitation Theory and Practice
2010;16(10):937-938
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo establish a new method for preparing synaptosomes.MethodsDensity gradient centrifugation method was used to isolate synaptosomes of mouse, checking by transmission electron microscopy.ResultsSynaptosomes prepared by this method had intact morphological characteristics, surrounding with a continuous oval-shaped membrane structure, moreover, mitochondrion and lots of synaptic vesicle in them.ConclusionThis method is applicable to establish a rapid, convenient and useful method for preparing synaptosomes.
