Comparison of Osteogenic Potential in vivo between Autologous Bone Marrow Mesenchymal Stem Cell Implanting and Fibrin Sealant Combined with Recombined Human Bone Morphogenetic Protein-2 Grafting
- VernacularTitle:自体骨髓间充质干细胞移植与纤维蛋白胶复合骨形态发生蛋白体内成骨的比较研究
- Author:
Yuli CHANG
;
Tiansheng SUN
;
Zhi LIU
;
Chaoqun YE
;
Zhaozhong YU
;
Zhicheng ZHANG
;
Dajiang REN
- Publication Type:Journal Article
- Keywords:
fibrin sealant, bone morphogenetic protein-2(BMP-2), bone marrow mesenchymal stem cells (BMSCs), cells implanting, bone defection
- From:
Chinese Journal of Rehabilitation Theory and Practice
2009;15(1):44-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the osteogenic potential for size-critical bone defect of fibrin sealant combined with recombined human bone morphogenetic protein-2 (rhBMP-2) grafting and varied autologous bone marrow mesenchymal stem cells (BMSCs) implanting in vivo. Methods BMSCs were cultured and induced with osteogenic supplement (OS) medium. BMSCs with and without OS induction were collected and percutaneously autologous injected respectively into the 15 mm bone defect of experimental rabbit model. The grafts were BMSCs, osteo-induced BMSCs, BMSCs and osteo-induced BMSCs, BMP combined with fibrin sealant, 0.9% NaCl solution. Osteogenesis at the defect area was assessed with regular radiography, histology and biomechanics. Results The FS/BMP group and the BMSCs+osteo-induced BMSCs group achieved complete bone healing with medullary cavity united, with the most new bone formation and the maximal load among those groups. Conclusion The osteogenic potential of both osteo-induced BMSCs combined with BMSCs and FS/BMP are similar, which are superior to that of BMSCs or osteo-induced BMSCs along.
