The Evaluation of Bone Regeneration Following Socket Preservation with Concentrated Growth Factor (CGF) and Poly Lactic-Co-Glycolic Acid (PLGA) Scaffold in Rabbits
10.21315/aos2022.17S1.OA02
- Author:
Nur Zety Mohd Noh
1
,
2
;
Nur Aliana Hidayah Mohamed
3
,
4
;
Erni Noor
5
Author Information
1. Centre of Periodontology Studies, Faculty of Dentistry, Universiti Teknologi MARA, 47000 Sungai Buloh, Selangor, Malaysia &
2. Department of Restorative Dentistry, Kulliyyah of Dentistry, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
3. Centre of Postgraduate Studies, Faculty of Dentistry, Universiti Teknologi MARA, 47000 Sungai Buloh, Selangor, Malaysia &
4. Centre of Preclinical Studies, Faculty of Dentistry, Universiti Teknologi MARA, 47000 Sungai Buloh, Selangor, Malaysia
5. Centre of Periodontology Studies, Faculty of Dentistry, Universiti Teknologi MARA, 47000 Sungai Buloh, Selangor, Malaysia
- Publication Type:Journal Article
- Keywords:
Concentrated growth factor;
Poly lactic-co-glycolic acid;
Regeneration;
Socket preservation
- MeSH:
Bone Regeneration;
Platelet-Derived Growth Factor;
Polylactic Acid-Polyglycolic Acid Copolymer
- From:Archives of Orofacial Sciences
2022;17(SUPP 1):35-50
- CountryMalaysia
- Language:English
-
Abstract:
ABSTRACT:Various grafting materials are utilised to facilitate regeneration. There is currently a paradigm shift
towards applying poly lactic-co-glycolic acid (PLGA), which is regarded as an excellent scaffold for tissue
engineering. Concentrated growth factor (CGF) has also been reported to promote wound healing.
Nevertheless, the role of PLGA microspheres as a substitute for bone graft material with CGF in bone
regeneration remains unclear. This study was designed to evaluate the effect of CGF with PLGA on
bone formation and the expression of alkaline phosphatase (ALP) following socket preservation. PLGA
microspheres were prepared using double solvent evaporation method and observed under scanning
electron microscopy (SEM). A 6 mL of rabbit’s blood was collected from the marginal ear vein and
centrifuged to obtain CGF. Blood was also collected for ALP assessment from 24 New Zealand White
(NZW) male rabbits subjected to the first upper left premolar extraction. Sockets were filled with CGF,
PLGA, CGF+PLGA or left empty and observed with microscopic computed tomography (micro-CT)
at four weeks and eight weeks. The SEM image revealed a spherical shape with interconnected pores
on the surface of the PLGA particles. Repeated measures ANOVA were used to evaluate the effect of
time and treatment (p < 0.05) with significant differences in bone width, height, volume, volume fraction
and expression of ALP was observed with CGF+PLGA. Both CGF and PLGA have the potential as the
alternative grafting materials and this study could serve as an ideal benchmark for future investigations on
the role of CGF+PLGA in bone regeneration enhancement.
- Full text:2.2022my0026.pdf
