In Vitro Evaluation of Human Demineralised Teeth Matrix on Osteogenic Differentiation of Gingival Mesenchymal Stem Cells
10.21315/aos2022.1702.OA08
- Author:
Dhanashree Deshpande
1
;
Arvind Karikal
1
;
Chethan Kumar
2
;
Basavarajappa Mohana Kumar
2
;
Veena Shetty
2
Author Information
1. Department of Oral and Maxillofacial Surgery, AB Shetty Memorial Institute of Dental Sciences, Nitte University, Deralakatte-575018, Mangaluru, India
2. Nitte University Centre for Stem Cell Research and Regenerative Medicine, KS Hegde Medical Academy, Nitte University, Deralakatte-575018, Mangaluru, India
- Publication Type:Journal Article
- Keywords:
Demineralised teeth matrix;
Gingival mesenchymal stem cells;
Human;
In vitro;
Osteogenesis
- MeSH:
Mesenchymal Stem Cells;
Dental Pulp--cytology;
Dentin
- From:Archives of Orofacial Sciences
2022;17(2):247-258
- CountryMalaysia
- Language:English
-
Abstract:
ABSTRACT:The use of tooth-derived material as a scaffold has gained attention recently due to its ease of availability
and bioactive properties. Hence, the objective of this study was to determine in vitro interaction of human
gingival mesenchymal stem cells (hGMSCs) with human demineralised teeth matrix (hDTM) on osteogenic
potential with or without osteogenic inducers. The hGMSCs were established and characterised on their
morphology, proliferation, population doubling time (PDT), viability, colony-forming ability, expression of
cell surface markers and adipogenic differentiation. Further, the effect of hDTM on the biocompatibility
and osteogenic differentiation ability of hGMSCs was evaluated. The hGMSCs displayed a fibroblast-like
appearance and exhibited a greater proliferative activity. The cells showed > 91% viability, and PDT varied
between 39.34 hours and 62.59 hours. Further, hGMSCs indicated their propensity to form clusters/
colonies, and expressed the markers, such as CD29, CD44, CD73 and CD90, but were negative for CD34
and CD45. When treated with adipogenic induction medium, hGMSCs were able to exhibit the formation
of neutral lipid vacuoles. The hGMSCs cultured with hDTM did not show any cytotoxic changes including
morphology and viability. Mineralisation of calcium nodules was observed in hGMSCs when cultured in
osteogenic induction (OI) medium as an indication of osteogenesis. hGMSCs when cultured with hDTM
confirmed the presence of a mineralised matrix. Further, when the cells were cultured with hDTM along
with OI, they showed slightly enhanced differentiation into osteocytes. In conclusion, hGMSCs were shown
to be biocompatible with hDTM, and demonstrated their enhanced osteogenic potential in the presence of
hDTM and osteogenic supplements.
- Full text:2.2022my00211.pdf
