In Vitro Cytotoxicity Analysis of Bioceramic Root Canal Sealers on Human Gingival Fibroblast Cells
10.21315/aos2022.1702.OA05
- Author:
Siti Aisyah Nadirah Ja’apar
1
;
Solachuddin Jauhari Arief Ichwan
2
;
Musliana Mustaffa
3
Author Information
1. Department of Biotechnology, Kulliyyah of Sciences, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
2. Dentistry Programme, Pengiran Anak Puteri Rashidah Saadatul Bolkiah Institute of Health Sciences, Universiti Brunei Darussalam, Jalan Tungku Link, BE1410 Brunei Darussalam
3. Department of Restorative Dentistry, Kulliyyah of Dentistry, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
- Publication Type:Journal Article
- Keywords:
Bioceramic root canal sealers;
Cell viability;
Cytotoxicity;
Endodontics;
Human gingival fibroblast cells
- MeSH:
Root Cause Analysis;
Dental Pulp Test
- From:Archives of Orofacial Sciences
2022;17(2):209-224
- CountryMalaysia
- Language:English
-
Abstract:
ABSTRACT:This study evaluated the cytotoxicity of four bioceramic root canal sealers (bioceramic sealers): GuttaFlow
Bioseal (GB), MTA Fillapex, CeraSeal Bioceramic root canal sealer (CS), and iRoot SP root canal sealer
(iRSP). The viability of human gingival fibroblast (HGF) cells was used to evaluate the cytotoxicity of these
bioceramic sealers. HGF cells were cultured and exposed to bioceramic sealer extracts for 24 hours, 48
hours and 72 hours at 37°C in an incubator humidified with 5% CO2. The 3-(4, 5-dimethylthiazol-2-yl)-2,
5-diphenyltetrazolium bromide or MTT assay was conducted to determine cell viability at each incubation
period and compared among all bioceramic sealers. The Kruskal-Wallis test revealed statistically significant
differences between the positive control group and MTA Fillapex, MTA Fillapex and GB, and between GB
and iRSP with p < 0.05. However, no statistical differences were found in cell viability for each material
across all the incubation periods. GB was the least cytotoxic bioceramic sealer with cell viability exceeding
90% throughout the 72-hour incubation followed by CS, iRSP, and MTA Fillapex with non-cytotoxicity
after 72-hour incubation, mild cytotoxicity after 72-hour incubation, and mild cytotoxicity after 72-hour
incubation, respectively. However, iRSP showed moderate cytotoxicity, and MTA Fillapex was severely
cytotoxic (< 30% cell viability) after 24-hour incubation.
- Full text:2.2022my0018.pdf
